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KMID : 0379119880160040210
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Korean Journal of Mycology
1988 Volume.16 No. 4 p.210 ~ p.213
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Studies on the Transfer of Isolated Nuclei from Pleurotus sapidus into Protoplasts of Pleurotus ostreatus
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Abstract
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Several reversion colonies were obtained after induced transfer of the isolated nuclei from P. sapidus into protoplast of P. ostreatus(Arg). These colonies showed three distinct cultural characteristics, type I produced spontaneous segregants of both parental types, type 2 showed segregants of non parental types, and type 3 gave rise to homogeneous colonies.
Isozyme patterns of esterase, malate dehydrogenase and superoxide dismutase showed substantial differences between parents and nuclei transferred strains. This observation supported that the isolated nuclei of P. sapidus were transferred into protoplast of P. ostreatus and expressed in recipient cell.
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